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Feb 1, 2012 · CATCATG3 (WT allele-specific), and 5CCGCTTCCATTGCTCA-. GCGG3 (mutant allele-specific) for bax. The sizes of the expected PCR products are ...
Jul 25, 2018 · ... CATCATG-3; NP cRNA, 5-AGTAGAAACAAGGGTA-. TTTTTC-3; and NP mRNA, oligo(dT). The following primers were used for qRT-PCR: NP, 5 ...
CATCATG-3 and 3-GCCTCCAACTCTACCTCTAGAGCCCCC-5 and cloned into pKHA3 (a gift from D. Brautigen). To prepare GFP-GSK3, GSK3 was subcloned from pKHA3 into ...
We examined the potential role of SMCs in regulating the shear stress–induced gene expression in ECs, using a parallel-plate coculture flow system in which ...
Jun 23, 2005 · CATCATG-3 and 5-CCAGCCATTAC-. GATGAAAGA-3). Samples were cycled at 93°C for 30 sec at the annealing tem- perature (sarcomeric -actinin, 57°C ...
Mar 20, 2008 · CATCATG-3′) and Bax-IN5 (5′-GTTGACCAGAGTGGCGTAGG-3′) for the Bax+/− targeted allele, Neo-R (5′-CCGCTTCCATTGCTCAGCGG-3′) and Bax IN5 for the ...
CATCATG-3' (the 31-bp inverted termi- nal repeat of the P element and with an outward orientation). 5 ' -CGCTCATTTCCGATGGTG-3 '. 5 ' -GGCTCCTTTGAACCCCAAG-3 ...
Jan 21, 2016 · We observed a delayed calpain activation that occurred downstream of mitochondrial engagement and directly preceded neuronal death. In contrast, ...
Jun 10, 2011 · CATCATG-3′), and loh3 (5′-TGGTTTAAAGAGAGGATTTTACGG-3′,. 5′-CAAAA TTTGTTCAGACTACAATTCAA-3′). Lipid Analysis. Free and total LCB analysis was ...
Feb 23, 2012 · 5'-CATCATG-3'. In this example, the two center-most bases (G and C) are complimentary, but the two sequences are not strictly palindromic ...